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Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration

  • Marina Dukhinova
  • Ekaterina Kopeikina
  • Eugene D. Ponomarev
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1745)

Abstract

The resident macrophages of the central nervous system (CNS), also known as microglia, and blood-derived macrophages play an important role in the functional activity of the normal CNS, as well as in the development of neuroinflammation during various neurodegenerative disorders. Microglia and macrophages represent heterogeneous populations, which can modulate CNS environment and have different effects on neuronal regeneration. In this chapter, the main features of microglial and macrophage subsets and current methods for investigation of their heterogeneity will be discussed.

Keywords

Microglia Macrophages Neuroinflammation Neurodegeneration 

Notes

Acknowledgments

The work was supported by Research Grant Council-Early Career Scheme grant reference 24100314 (Hong Kong) to E.D.P.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Marina Dukhinova
    • 1
  • Ekaterina Kopeikina
    • 1
  • Eugene D. Ponomarev
    • 1
  1. 1.School of Biomedical Sciences, Faculty of MedicineThe Chinese University of Hong KongHong KongChina

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