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Plant Senescence pp 65–80Cite as

Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1744))

Abstract

Leguminous crops can form nodules to fix atmospheric nitrogen (N2). Senescence of nodules is associated with a rapid decline in N fixation. During the process of nodule senescence, a number of visible or detectable changes on morphology, biochemistry, and physiology occur. Here we describe several methods for examining the senescing phenotypes of nodules, including rhizobium inoculation, nitrogenase activity determination with the acetylene reduction assay, leghemoglobin content determination, and apoptotic cell identification with TdT-mediated dUTP-biotin nick end-labeling (TUNEL) staining.

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Authors and Affiliations

  1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning, China

    Xiuying Xia

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  1. Xiuying Xia
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Correspondence to Xiuying Xia .

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Editors and Affiliations

  1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, China

    Yongfeng Guo

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Xia, X. (2018). Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7672-0_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7670-6

  • Online ISBN: 978-1-4939-7672-0

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