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Study of Hydrogen Peroxide as a Senescence-Inducing Signal

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Plant Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1744))

Abstract

In many plant species, leaf senescence correlates with an increase in intracellular levels of reactive oxygen species (ROS) as well as differential regulation of anti-oxidative systems. Due to their reactive nature, reactive oxygen species (ROS) were considered to have only detrimental effects for long time. However, ROS turned out to be more than just toxic by-products of aerobic metabolism but rather major components in different signaling pathways. Considering its relatively long half-life, comparably low reactivity, and its ability to cross membranes, especially hydrogen peroxide, has gained attention as a signaling molecule. In this article, a set of tools to study hydrogen peroxide contents and the activity of its scavenging enzymes in correlation with leaf senescence parameters is presented.

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Authors and Affiliations

  1. ZMBP, Department of General Genetics, University Tuebingen, Tuebingen, Germany

    Stefan Bieker, Maren Potschin & Ulrike Zentgraf

Authors
  1. Stefan Bieker
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  2. Maren Potschin
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  3. Ulrike Zentgraf
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Corresponding author

Correspondence to Ulrike Zentgraf .

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Editors and Affiliations

  1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, China

    Yongfeng Guo

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Bieker, S., Potschin, M., Zentgraf, U. (2018). Study of Hydrogen Peroxide as a Senescence-Inducing Signal. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7672-0_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7670-6

  • Online ISBN: 978-1-4939-7672-0

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