Abstract
High salinity, one of the most severe abiotic stresses encountered by land plants, often results from water deficit and also induces whole-plant senescence. Thus, salt treatment provides a useful technique for stress-mediated induction of senescence in plants. In this chapter, we describe the procedures to induce senescence in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), using NaCl or KCl. Furthermore, we present experimental approaches to measure salt stress-induced leaf senescence.
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References
Lim PO, Kim HJ, Nam HG (2007) Leaf senescence. Annu Rev Plant Biol 58:115–136
Lutts S, Kinet JM, Bouharmont J (1996) NaCl-induced senescence in leaves of rice (Oryza sativa L) cultivars differing in salinity resistance. Ann Bot 78:389–398
Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111
Quirino BF, Normanly J, Amasino RM (1999) Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes. Plant Mol Biol 40:267–278
Lutts S, Kinet JM, Bouharmont J (1995) Changes in plant response to NaCl during development of rice (Oryza sativa L) varieties differing in salinity resistance. J Exp Bot 46:1843–1852
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
Weaver LM, Amasino RM (2001) Senescence is induced in individually darkened Arabidopsis leaves but inhibited in whole darkened plants. Plant Physiol 127:876–886
Balazadeh S, Siddiqui H, Allu AD et al (2010) A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence. Plant J 62:250–264
Kim YS, Sakuraba Y, Han SH et al (2013) Mutation of the Arabidopsis NAC016 transcription factor delays leaf senescence. Plant Cell Physiol 54:1660–1672
Thompson AR, Doelling JH, Suttangkakul A et al (2005) Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol 138:2097–2110
Sakuraba Y, Lee SH, Kim YS et al (2014) Delayed degradation of chlorophylls and photosynthetic proteins in Arabidopsis autophagy mutants during stress-induced leaf yellowing. J Exp Bot 65:3915–3925. https://doi.org/10.1093/jxb/eru008
Santos CL, Campos A, Azevedo H et al (2001) In situ and in vitro senescence induced by KCl stress: nutritional imbalance, lipid peroxidation and antioxidant metabolism. J Exp Bot 52:351–360
Kim JH, Woo HR, Kim J et al (2009) Trifurcate feed-forward regulation of age-dependent cell death involving miR164 in Arabidopsis. Science 323:1053–1057
Ghanem ME, Albacete A, Martinez-Andujar C et al (2008) Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.) J Exp Bot 59:3039–3050
Lohman KN, Gan SS, John MC et al (1994) Molecular analysis of natural leaf senescence in Arabidopsis thaliana. Physiol Plant 92:322–328
Oh SA, Lee SY, Chung IK et al (1996) A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescence. Plant Mol Biol 30:739–754
Guo YF, Gan SS (2006) AtNAP, a NAC family transcription factor, has an important role in leaf senescence. Plant J 46:601–612
Allu AD, Soja AM, Wu A et al (2014) Salt stress and senescence: identification of cross-talk regulatory components. J Exp Bot 65:3993–4008. https://doi.org/10.1093/jxb/eru173
Acknowledgments
This work was carried out with the support of Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ011079), Rural Development Administration, Republic of Korea.
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Sakuraba, Y., Kim, D., Paek, NC. (2018). Salt Treatments and Induction of Senescence. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_13
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DOI: https://doi.org/10.1007/978-1-4939-7672-0_13
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