Abstract
Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of H2O2 and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes. Deciphering the time and spatial regulation of ROS and NO is very important to establish the cellular response of plants to adverse conditions. This chapter is mainly focused on the imaging of ROS and RNS accumulation in vivo in plant tissues undergoing PCD.
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Acknowledgments
This work was financially supported by European Regional Development Fund cofinanced grant (BIO2015-67657-P) with the Ministry of Economy, Industry and Competitiveness; and the Junta de Andalucía (group no. BIO-337 and grant P12-BIO-296). L.C. T-C was supported by a fellowship for Academic Staff (FPU) from the Government of Spain-Ministry of Education, Culture, and Sports. The confocal laser fluorescence microscopic analyses were carried out at the Technical Services of the EEZ (CSIC).
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Terrón-Camero, L.C., Molina-Moya, E., Sanz-Fernández, M., Sandalio, L.M., Romero-Puertas, M.C. (2018). Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death. In: De Gara, L., Locato, V. (eds) Plant Programmed Cell Death. Methods in Molecular Biology, vol 1743. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7668-3_9
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DOI: https://doi.org/10.1007/978-1-4939-7668-3_9
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