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Measurement of Hypersensitive Cell Death Triggered by Avirulent Bacterial Pathogens in Arabidopsis

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Plant Programmed Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1743))

Abstract

The hypersensitive response is one of the most powerful and complex defense reactions to survive to pathogen attacks during an incompatible plant–pathogen interaction. Local programmed cell death accompanies the hypersensitive response at the site of infection to prevent pathogen growth and spread. A precise quantitative assessment of this form of programmed cell death is essential to unravel the genetic and molecular mechanisms underlying the process. Here, we first describe the optimization of a Trypan Blue staining protocol for quantitatively measuring the HR-cell death in Arabidopsis. Furthermore, we provide an electrolyte leakage protocol based on pathogen vacuum infiltration, which allows its simultaneous application to a large number of plants as well as to Arabidopsis mutants affected by small size phenotype.

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Authors and Affiliations

  1. Department of Biotechnology, University of Verona, Verona, Italy

    Zahra Imanifard, Elodie Vandelle & Diana Bellin

Authors
  1. Zahra Imanifard
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  2. Elodie Vandelle
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  3. Diana Bellin
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Corresponding author

Correspondence to Diana Bellin .

Editor information

Editors and Affiliations

  1. Food Sciences and Human Nutrition Unit, Università Campus Bio-Medico di Roma, Roma, Italy

    Laura De Gara

  2. Food Sciences and Human Nutrition Unit, Università Campus Bio-Medico di Roma, Roma, Italy

    Vittoria Locato

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Imanifard, Z., Vandelle, E., Bellin, D. (2018). Measurement of Hypersensitive Cell Death Triggered by Avirulent Bacterial Pathogens in Arabidopsis. In: De Gara, L., Locato, V. (eds) Plant Programmed Cell Death. Methods in Molecular Biology, vol 1743. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7668-3_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7668-3_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7667-6

  • Online ISBN: 978-1-4939-7668-3

  • eBook Packages: Springer Protocols

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