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Plant Programmed Cell Death pp 9–19Cite as

Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1743))

Abstract

The investigation of autophagy particularly when observed during programmed cell death (PCD) is strongly based on the morphological features recorded with transmission electron microscope (TEM). Here we describe methods to induce and to inhibit autophagy in plants. Also some tips for obtaining better preservation of biological membranes, crucial for the investigation of autophagy, are provided together with information about plant autophagic mutants, use of antibodies and methods for 3D reconstruction of large membrane-bound objects that are commonly formed during autophagic processes leading to PCD in plants.

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Author information

Authors and Affiliations

  1. Department of Biology, University of Florence, Florence, Italy

    Alessio Papini

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  1. Alessio Papini
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Correspondence to Alessio Papini .

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Editors and Affiliations

  1. Food Sciences and Human Nutrition Unit, Università Campus Bio-Medico di Roma, Roma, Italy

    Laura De Gara

  2. Food Sciences and Human Nutrition Unit, Università Campus Bio-Medico di Roma, Roma, Italy

    Vittoria Locato

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Papini, A. (2018). Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death. In: De Gara, L., Locato, V. (eds) Plant Programmed Cell Death. Methods in Molecular Biology, vol 1743. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7668-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7668-3_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7667-6

  • Online ISBN: 978-1-4939-7668-3

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