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Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues

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Hypoxia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1742))

Abstract

Hypoxia (insufficient O2 availability) is involved in various biological processes, such as tumorigenesis and inflammation. Hypoxia results in stabilization of hypoxia-inducible factors (HIFs) including HIF1α and HIF2α. Here we describe a protocol to detect mouse and human tissue hypoxia by using Hypoxyprobe and immunohistochemical staining for HIF1α and HIF2α.

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References

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Acknowledgments

This work was supported by funding from the National Heart, Lung, and Blood Institute (grant number HL66310).

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Author notes

  1. Fuming Li and Kyoung Eun Lee contributed equally to this work.

Authors and Affiliations

  1. Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA, USA

    Fuming Li, Kyoung Eun Lee & M. Celeste Simon

  2. Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA

    Fuming Li, Kyoung Eun Lee & M. Celeste Simon

Authors
  1. Fuming Li
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  2. Kyoung Eun Lee
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  3. M. Celeste Simon
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Corresponding author

Correspondence to M. Celeste Simon .

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Editors and Affiliations

  1. Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, Utah, USA

    L. Eric Huang

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Li, F., Lee, K.E., Simon, M.C. (2018). Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_24

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  • DOI: https://doi.org/10.1007/978-1-4939-7665-2_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7664-5

  • Online ISBN: 978-1-4939-7665-2

  • eBook Packages: Springer Protocols

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