Abstract
Aldehyde dehydrogenase and mammosphere assays enable the cost-effective quantification and characterization of cancer stem cells (CSCs) from cancer cell lines as well as cancer tissue. Here we describe the quantification of CSCs in breast cancer cell lines using aldehyde dehydrogenase and mammosphere assays under hypoxic (1% O2) and non-hypoxic (20% O2) culture conditions. Using this method, a significant enrichment of CSCs compared to bulk populations is observed when breast cancer cells are exposed to 1% O2 for 72 h.
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Acknowledgments
Cancer research in the authors’ laboratory is supported by grants from the American Cancer Society, Armstrong Family Foundation, Department of Defense Breast Cancer Research Program, and the Cindy Rosencrans Fund for Triple Negative Breast Cancer. G.L.S. is an American Cancer Society Research Professor and the C. Michael Armstrong Professor at the Johns Hopkins University School of Medicine.
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Samanta, D., Semenza, G.L. (2018). In Vitro Assays of Breast Cancer Stem Cells. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_21
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DOI: https://doi.org/10.1007/978-1-4939-7665-2_21
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