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Testing Acute Oxygen Sensing in Genetically Modified Mice: Plethysmography and Amperometry

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Hypoxia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1742))

Abstract

Monitoring responsiveness to acute hypoxia of whole animals and single cells is essential to investigate the nature of the mechanisms underlying oxygen (O2) sensing. Here we describe the protocols followed in our laboratory to evaluate the ventilatory response to hypoxia in normal and genetically modified animals. We also describe the amperometric technique used to monitor single-cell catecholamine release from chemoreceptor cells in carotid body and adrenal medulla slices.

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Acknowledgments

This work was supported by the Botín Foundation, the Spanish Ministry of Economy and Innovation (SAF2012-39343, SAF2016-74990-R), and the European Research Council (ERC Advanced Grant PRJ201502629).

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Authors and Affiliations

  1. Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain

    Patricia Ortega-Sáenz, Candela Caballero, Lin Gao & José López-Barneo

  2. Departamento de Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, Seville, Spain

    Patricia Ortega-Sáenz, Candela Caballero, Lin Gao & José López-Barneo

  3. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain

    Patricia Ortega-Sáenz, Candela Caballero, Lin Gao & José López-Barneo

  4. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Candela Caballero

Authors
  1. Patricia Ortega-Sáenz
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  2. Candela Caballero
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  3. Lin Gao
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  4. José López-Barneo
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Corresponding authors

Correspondence to Patricia Ortega-Sáenz or José López-Barneo .

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, Utah, USA

    L. Eric Huang

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Ortega-Sáenz, P., Caballero, C., Gao, L., López-Barneo, J. (2018). Testing Acute Oxygen Sensing in Genetically Modified Mice: Plethysmography and Amperometry. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7665-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7664-5

  • Online ISBN: 978-1-4939-7665-2

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