Abstract
Carotid bodies are sensory organs for monitoring chemical composition of the arterial blood, especially the O2 levels. Carotid bodies are located bilaterally at the bifurcation of the common carotid artery. Hypoxia increases sensory nerve activity of the carotid body, which is transmitted to the brainstem neurons triggering reflex stimulation of breathing and blood pressure. Measurement of action potentials from the carotid sinus nerve is a widely used approach for understanding the mechanisms of hypoxic sensing by the carotid body. Here, we describe the detailed methodology for recording action potential signals from the carotid sinus nerve from in vivo and ex vivo carotid bodies from rats.
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References
Kumar P, Prabhakar NR (2012) Peripheral chemoreceptors: function and plasticity of the carotid body. Compr Physiol 2:141–219
Prabhakar NR (2006) O2 sensing at the mammalian carotid body: why multiple O2 sensors and multiple transmitters? Exp Physiol 91(1):17–23. https://doi.org/10.1113/expphysiol.2005.031922
Peng Y-J, Nanduri J, Raghuraman G, Souvannakitti D, Gadalla MM, Kumar GK, Snyder SH, Prabhakar NR (2010) H2S mediates O2 sensing in the carotid body. Proc Natl Acad Sci U S A 107(23):10719–10724. https://doi.org/10.1073/pnas.1005866107
Conde SV, Obeso A, Rigual R, Monteiro EC, Gonzalez C (2006) Function of the rat carotid body chemoreceptors in ageing. J Neurochem 99(3):711–723. https://doi.org/10.1111/j.1471-4159.2006.04094.x
Acknowledgments
This work was supported by National Institutes of Health grant PO1-HL- 90554.
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Peng, YJ., Prabhakar, N.R. (2018). Measurement of Sensory Nerve Activity from the Carotid Body. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_11
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DOI: https://doi.org/10.1007/978-1-4939-7665-2_11
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