Abstract
We describe an isolation method of tumor-infiltrating lymphocytes (TILs) from glioblastoma tumors for the purpose of analysis by flow cytometry. This protocol is unique from many others in that the use of a selective lymphocyte isolation procedure, such as a Ficoll or Percoll gradient, is not used. We find that staining of TILs and analysis by flow cytometry is not affected by the presence of heterogeneous populations, while other selective isolation procedures can significantly decrease lymphocyte yield from already rare populations.
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Woroniecka, K., Chongsathidkiet, P., Elsamadicy, A., Farber, H., Cui, X., Fecci, P.E. (2018). Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. In: Placantonakis, D. (eds) Glioblastoma. Methods in Molecular Biology, vol 1741. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7659-1_18
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DOI: https://doi.org/10.1007/978-1-4939-7659-1_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7658-4
Online ISBN: 978-1-4939-7659-1
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