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Orthotopic Patient-Derived Glioblastoma Xenografts in Mice

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Glioblastoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1741))

Abstract

Patient-derived xenografts (PDX) provide in vivo glioblastoma (GBM) models that recapitulate actual tumors. Orthotopic tumor xenografts within the mouse brain are obtained by injection of GBM stem-like cells derived from fresh surgical specimens. These xenografts reproduce GBM’s histologic complexity and hallmark biological behaviors, such as brain invasion, angiogenesis, and resistance to therapy. This method has become essential for analyzing mechanisms of tumorigenesis and testing the therapeutic effect of candidate agents in the preclinical setting. Here, we describe a protocol for establishing orthotopic tumor xenografts in the mouse brain with human GBM cells.

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Authors and Affiliations

  1. Department of Neurosurgery, New York University School of Medicine, New York, NY, USA

    Zhongye Xu, Michael Kader, Rajeev Sen & Dimitris G. Placantonakis

  2. Kimmel Center for Stem Cell Biology, New York University School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  3. Perlmutter Cancer Center, New York University School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  4. Brain Tumor Center, New York University School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  5. Neuroscience Institute, New York University School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

Authors
  1. Zhongye Xu
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  2. Michael Kader
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  3. Rajeev Sen
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  4. Dimitris G. Placantonakis
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Corresponding author

Correspondence to Dimitris G. Placantonakis .

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Editors and Affiliations

  1. Department of Neurosurgery, NYU School of Medicine, New York, New York, USA

    Dimitris G. Placantonakis

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Xu, Z., Kader, M., Sen, R., Placantonakis, D.G. (2018). Orthotopic Patient-Derived Glioblastoma Xenografts in Mice. In: Placantonakis, D. (eds) Glioblastoma. Methods in Molecular Biology, vol 1741. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7659-1_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7659-1_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7658-4

  • Online ISBN: 978-1-4939-7659-1

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