Abstract
The study of extracellular RNA has been recently reported as a tool for biomarker discovery. Extracellular vesicles can be isolated from different types of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.
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Acknowledgments
This work was supported by the Office of the Director, National Institutes of Health (USA) through award UH3 TR000884. We acknowledge the expert assistance of Caitlyn Foerst and thank the members of our laboratories for their contributions.
Disclosures: None.
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Yan, I.K., Berdah, V.X., Patel, T. (2018). Isolation of Extracellular RNA from Bile. In: Patel, T. (eds) Extracellular RNA. Methods in Molecular Biology, vol 1740. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7652-2_6
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DOI: https://doi.org/10.1007/978-1-4939-7652-2_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7651-5
Online ISBN: 978-1-4939-7652-2
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