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Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve

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Schwann Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1739))

Abstract

Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.

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References

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Author information

Authors and Affiliations

  1. Vollum Institute, Oregon Health & Science University, Portland, OR, USA

    Kelly R. Monk

  2. Department of Developmental Biology, Washington University in St. Louis, St. Louis, MO, USA

    Rebecca L. Cunningham & Kelly R. Monk

Authors
  1. Rebecca L. Cunningham
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  2. Kelly R. Monk
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Corresponding author

Correspondence to Kelly R. Monk .

Editor information

Editors and Affiliations

  1. Department of Neurological Surgery, University of Miami, Miami, Florida, USA

    Paula V. Monje

  2. Department of Biological Sciences, Rutgers University, Newark, New Jersey, USA

    Haesun A. Kim

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Cunningham, R.L., Monk, K.R. (2018). Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve. In: Monje, P., Kim, H. (eds) Schwann Cells. Methods in Molecular Biology, vol 1739. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7649-2_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7649-2_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7648-5

  • Online ISBN: 978-1-4939-7649-2

  • eBook Packages: Springer Protocols

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