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Metabolic Profiling pp 99–116Cite as

LC-MS Untargeted Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1738))

Abstract

LC-MS untargeted analysis is a valuable tool in the field of metabolic profiling (metabonomics/metabolomics), and the applications of this technology have grown rapidly over the past decade. LC-MS offers advantages over other analytical platforms such as speed, sensitivity, relative ease of sample preparation, and large dynamic range. As with any analytical approach, there are still drawbacks and challenges to overcome, but advances are constantly being made regarding both column chemistries and instrumentation. There are numerous untargeted LC-MS approaches which can be used in this ever-growing research field; these can be optimized depending on sample type and the nature of the study or biological question. Some of the main LC-MS approaches for the untargeted analysis of biological samples will be described in detail in the following protocol.

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Author information

Authors and Affiliations

  1. Computational and Systems Medicine, Imperial College London, London, UK

    Elizabeth J. Want

Authors
  1. Elizabeth J. Want
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Correspondence to Elizabeth J. Want .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Georgios A. Theodoridis

  2. School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Helen G. Gika

  3. Department of Surgery and Cancer, Imperial College London, London, United Kingdom

    Ian D. Wilson

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Want, E.J. (2018). LC-MS Untargeted Analysis. In: Theodoridis, G., Gika, H., Wilson, I. (eds) Metabolic Profiling. Methods in Molecular Biology, vol 1738. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7643-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7643-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7642-3

  • Online ISBN: 978-1-4939-7643-0

  • eBook Packages: Springer Protocols

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