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HILIC-MS/MS Multi-Targeted Method for Metabolomics Applications

  • Christina Virgiliou
  • Helen G. Gika
  • Georgios A. Theodoridis
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1738)

Abstract

Metabolomics aims at the identification and quantification of key-end point metabolites, basically polar, in order to study changes in biochemical activities in response to pathophysiological stimuli or genetic modifications. Targeted profiling assays have enjoyed a growing popularity during the last years with LC-MS/MS as a powerful tool for development of such (semi-) quantitative methods for a large number of metabolites. Here we describe a method for absolute quantification of ca. 100 metabolites belonging to key metabolite classes such as sugars, amino acids, nucleotides, organic acids, and amines with a hydrophilic interaction liquid chromatography (HILIC) system comprised of ultra (high) performance liquid chromatography (UHPLC) with detection on a triple-quadrupole mass spectrometer operating in both positive and negative electrospray ionization modes.

Key words

HILIC-MS/MS Metabolic profiling Targeted metabolomics Polar analytes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Christina Virgiliou
    • 1
  • Helen G. Gika
    • 2
  • Georgios A. Theodoridis
    • 1
  1. 1.Laboratory of Forensic Medicine and Toxicology, Department of ChemistryAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Department of MedicineAristotle UniversityThessalonikiGreece

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