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Quality Control and Validation Issues in LC-MS Metabolomics

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Metabolic Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1738))

Abstract

Global metabolic profiling (untargeted metabolomics) of different and complex biological matrices aims to implement an holistic, hypothesis-free analysis of (potentially) all the metabolites present in the analyzed sample. However, such an approach, although it has been the focus of great interest over the past few years, still faces many limitations and challenges, particularly with regard to the validation and the quality of the obtained results. The present protocol describes a quality control (QC) procedure for monitoring the precision of the analytical process involving untargeted metabolic phenotyping of urine and plasma/serum. The described/suggested methodology can be applied to different biological matrices, such as biological biofluids, cell, and tissue extracts.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Aristotle University, Thessaloniki, Greece

    Olga Begou & Georgios A. Theodoridis

  2. School of Medicine, Aristotle University, Thessaloniki, Greece

    Helen G. Gika

  3. Department of Surgery and Cancer, Imperial College London, London, UK

    Ian D. Wilson

Authors
  1. Olga Begou
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  2. Helen G. Gika
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  3. Georgios A. Theodoridis
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  4. Ian D. Wilson
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Corresponding author

Correspondence to Helen G. Gika .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Georgios A. Theodoridis

  2. School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Helen G. Gika

  3. Department of Surgery and Cancer, Imperial College London, London, United Kingdom

    Ian D. Wilson

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Begou, O., Gika, H.G., Theodoridis, G.A., Wilson, I.D. (2018). Quality Control and Validation Issues in LC-MS Metabolomics. In: Theodoridis, G., Gika, H., Wilson, I. (eds) Metabolic Profiling. Methods in Molecular Biology, vol 1738. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7643-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7643-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7642-3

  • Online ISBN: 978-1-4939-7643-0

  • eBook Packages: Springer Protocols

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