Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation

  • Aikaterina Siopi
  • Vassilis Mougios
Part of the Methods in Molecular Biology book series (MIMB, volume 1738)


Metabolomics can be of great value in the study of exercise metabolism. However, because of the high intraindividual and interindividual biological variability of the human metabolome, special considerations should be taken into account when designing an acute-exercise metabolomic study. To study different exercise parameters, e.g., different exercise modes, intensities, etc., a crossover study design, where each participant acts as their own control, is preferable to a parallel design, one involving different groups of participants. Moreover, the study should include a no exercise, control trial. Before each trial, participants should follow carefully designed preparatory steps to control for possible confounding factors, i.e., maintain repeatable and constant conditions for all individual trials of the study to minimize variation due to factors other than the one(s) being studied. This chapter focuses on the design of human metabolomic studies, where the intervention is an acute metabolic challenge, such as an exercise bout or a test meal, and presents some basic steps for screening potential participants, performing preliminary tests, preparing for the trial day, and performing the trial.

Key words

Metabolomics Acute exercise Study design Humans 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Physical Education and Sport Science at ThessalonikiAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Department of Physical Education and Sport Science at ThermiAristotle University of ThessalonikiThessalonikiGreece

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