UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)

  • Federico Ponzetto
  • Julien Boccard
  • Raul Nicoli
  • Tiia Kuuranne
  • Martial Saugy
  • Serge Rudaz
Part of the Methods in Molecular Biology book series (MIMB, volume 1738)


The extraction and untargeted UHPLC-HRMS analysis of endogenous steroids in serum samples is described in this protocol. The employed full-scan acquisition mode provides the adequate sensitivity to highlight the main endogenous steroids present in blood, including mineralocorticoids, progestogens, and androgens. Technical aspects for both chromatography and mass spectrometry are discussed in detail, together with a proposition of setup for sample sequence and data analysis. Furthermore, general comments are given to help the assessment of data quality and system performance.

Key words

Steroids Extended steroid profiles Steroidomics UHPLC-HRMS 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Federico Ponzetto
    • 1
  • Julien Boccard
    • 2
    • 3
  • Raul Nicoli
    • 1
  • Tiia Kuuranne
    • 1
  • Martial Saugy
    • 4
  • Serge Rudaz
    • 2
    • 3
  1. 1.Swiss Laboratory for Doping AnalysesUniversity Center of Legal Medicine Geneva and Lausanne, Centre Hospitalier Universitaire Vaudois and University of LausanneLausanneSwitzerland
  2. 2.School of Pharmaceutical SciencesUniversity of Geneva, University of LausanneGeneva 4Switzerland
  3. 3.Swiss Center of Applied Human Toxicology (SCAHT)University of BaselBaselSwitzerland
  4. 4.Center of Research and Expertise in Anti-Doping SciencesUniversity of LausanneLausanneSwitzerland

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