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NMR Spectroscopy Protocols for Food Metabolomics Applications

  • Evangelia Ralli
  • Maria Amargianitaki
  • Efi Manolopoulou
  • Maria Misiak
  • Georgios Markakis
  • Sofia Tachtalidou
  • Alexandra Kolesnikova
  • Photis Dais
  • Apostolos Spyros
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1738)

Abstract

NMR spectroscopy has become an indispensable tool for the metabolic profiling of foods and food products. In the present protocol, we report an analytical approach based on liquid-state NMR for the determination of polar and nonpolar metabolites in some common liquid (wine, spirits, juice) and solid (cheese, coffee, honey) foods. Although the diversity of foods precludes the use of a single protocol, with small modifications, the proposed methodologies can be adapted to a broader range of foodstuffs.

Key words

NMR spectroscopy Metabolite profiling Food analysis Authentication Quality control 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Evangelia Ralli
    • 1
  • Maria Amargianitaki
    • 1
  • Efi Manolopoulou
    • 1
  • Maria Misiak
    • 1
  • Georgios Markakis
    • 1
  • Sofia Tachtalidou
    • 1
  • Alexandra Kolesnikova
    • 1
  • Photis Dais
    • 1
  • Apostolos Spyros
    • 1
  1. 1.NMR Laboratory, Chemistry DepartmentUniversity of CreteHeraklionGreece

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