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Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR

  • Jan Schripsema
  • Denise Dagnino
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1738)

Abstract

Metabolomics is the area of research, which strives to obtain complete metabolic fingerprints, to detect differences between them, and to provide hypothesis to explain those differences [1]. But obtaining complete metabolic fingerprints is not an easy task. Metabolite extraction is a key step during this process, and much research has been devoted to finding the best solvent mixture to extract as much metabolites as possible.

Here a procedure is described for analysis of both polar and apolar metabolites using a two-phase extraction system. D2O and CDCl3 are the solvents of choice, and their major advantage is that, for the identification of the compounds, standard databases can be used because D2O and CDCl3 are the solvents most commonly used for pure compound NMR spectra. The procedure enables the absolute quantification of components via the addition of suitable internal standards. The extracts are also suitable for further analysis with other systems like LC-MS or GC-MS.

Key words

Two-phase extraction NMR Metabolic fingerprints Plants Identification Quantification 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jan Schripsema
    • 1
  • Denise Dagnino
    • 1
  1. 1.Grupo MetabolômicaUniversidade Estadual do Norte FluminenseCampos dos GoytacazesBrazil

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