Abstract
Besides their well-known posttranscriptional effects on mRNA translation and decay, sRNAs and associated RNA chaperones (e.g., Hfq, CsrA) sometimes regulate gene expression at the transcriptional level. In this case, the sRNA-dependent machinery modulates the activity of the transcription termination factor Rho, a ring-shaped RNA translocase/helicase that dissociates transcription elongation complexes at specific loci of the bacterial genome. Here, we describe biochemical assays to detect Rho-dependent termination signals in genomic regions of interest and to assess the effects of sRNAs and/or associated RNA chaperones on such signals.
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Acknowledgments
This work was supported by a PhD scholarship from Région Centre Val-de-Loire to C.N. and by a grant from Agence Nationale de la Recherche (ANR-15-CE11-0024-02) and CNRS core funding to M.B.
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Nadiras, C., Schwartz, A., Delaleau, M., Boudvillain, M. (2018). Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro. In: Arluison, V., Valverde, C. (eds) Bacterial Regulatory RNA. Methods in Molecular Biology, vol 1737. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7634-8_7
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DOI: https://doi.org/10.1007/978-1-4939-7634-8_7
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