Organoids as a Model to Study Infectious Disease
The advent of the gastric organoid culture system has provided a new model to emulate native epithelial tissue in vitro. Gastric organoids grow from isolated epithelial stem cells and develop into three dimensional structures that can be used to study host physiology. Here we describe current laboratory protocols for growing gastric organoids and the microinjection of pathogens such as Helicobacter pylori into the lumen of gastric organoids in order to study the cellular response following infection.
Key wordsGastric organoids Microinjection Pathogens Helicobacter pylori Fluorescent dye
This work was supported by National Institutes of Health Grants RO1 DK102551 (MHM). This project was supported in part by PHS Grant P30 DK078392 (Live Microscopy Core at University of Cincinnati) of the Digestive Disease Research Core Center in Cincinnati.