Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection
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Bioluminescence is the process of production and emission of light by a living organism, usually as the by-product of the oxidative enzyme, luciferase. Currently available technology allows for the exploitation of a bioluminescent reporter system to study bacterial gene regulation during rodent infection, in real time, over a large dynamic range. Here we show how this imaging system can be used to study virulence gene regulation during Salmonella enterica infection in the mouse model. To demonstrate this technique we show the ex vivo expression pattern of the gene dksA, encoding a conserved and pleotropic regulator, which plays a key role in Salmonella pathogenicity .
Key wordsLuciferase Bioluminescence Reporter gene In vivo imaging Transcriptional regulation Host infection
The research in Gal-Mor lab is supported by a grant number 1096.39.11/2010 from the German-Israeli Foundation for Scientific Research and Development (GIF); by a grant number 999/14 from the Israel Science Foundation (ISF) and by grant number 3-0000-12435 from Infect-ERA and the Chief Scientist’s Bureau in the Israeli Ministry of Health.