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A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean

  • Ángeles Hidalgo
  • José E. Ruiz-Sainz
  • José M. VinardellEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1734)

Abstract

Plants use long-distance signaling mechanisms to coordinate their growth and control their interactions, positive or negative, with microbes. Split-root systems (SRS) have been used to study the relevance of both local and systemic plant mechanisms that participate in the control of rhizobia–legume symbioses. In this work we have developed a modification of the standard split-root system (SRS) used with soybean. This modified method, unlike previous systems, operates in hydroponics conditions and therefore is nondestructive and allows for the continuous monitoring of soybean roots throughout the whole experiment.

Key words

Soybean Split-root system Nodulation Autoregulation of nodulation Hydroponic culture 

Notes

Acknowledgment

This work was supported by grants from the Spanish Ministry of Science and Innovation (BIO2011-30229) and the Andalusia Government (P07-CVI-07500).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Ángeles Hidalgo
    • 1
  • José E. Ruiz-Sainz
    • 1
  • José M. Vinardell
    • 1
    Email author
  1. 1.Department of Microbiology, Faculty of BiologyUniversity of SevilleSevilleSpain

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