A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean
Plants use long-distance signaling mechanisms to coordinate their growth and control their interactions, positive or negative, with microbes. Split-root systems (SRS) have been used to study the relevance of both local and systemic plant mechanisms that participate in the control of rhizobia–legume symbioses. In this work we have developed a modification of the standard split-root system (SRS) used with soybean. This modified method, unlike previous systems, operates in hydroponics conditions and therefore is nondestructive and allows for the continuous monitoring of soybean roots throughout the whole experiment.
Key wordsSoybean Split-root system Nodulation Autoregulation of nodulation Hydroponic culture
This work was supported by grants from the Spanish Ministry of Science and Innovation (BIO2011-30229) and the Andalusia Government (P07-CVI-07500).
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