This chapter describes the steps needed to inoculate host plants with a fungus of interest, and subsequently to visualize the infection using confocal microscopy. As an exemplar, we consider the interaction between wheat and the Septoria leaf blotch fungus, Zymoseptoria tritici. This method is easiest when a GFP- or other fluorophore-tagged strain of the studied fungus is available, but notes are also provided which describe possible staining techniques which may be employed if fluorescent fungus is unavailable in your system.
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
Gong X, Hurtado O, Wang B et al (2015) pFPL vectors for high-throughput protein localization in fungi: detecting cytoplasmic accumulation of putative effector proteins. Mol Plant-Microbe Interact 28:107–121CrossRefPubMedGoogle Scholar
Gupta YK, Dagdas YF, Martinez-Rocha AL et al (2015) Septin-dependent assembly of the exocyst is essential for plant infection by Magnaporthe oryzae. Plant Cell 27:3277–3289CrossRefPubMedPubMedCentralGoogle Scholar
Kilaru S, Schuster M, Studholme D et al (2015) A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici. Fungal Genet Biol 79:125–131CrossRefPubMedPubMedCentralGoogle Scholar
Keon J, Antoniw J, Carzaniga R et al (2007) Transcriptional adaptation of Mycosphaerella graminicola to programmed cell death (PCD) of its susceptible wheat host. Mol Plant-Microbe Interact 20:178–193CrossRefPubMedGoogle Scholar
Littlejohn GR, Gouveia JD, Edner C et al (2010) Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll. New Phytol 186:1018–1025CrossRefPubMedGoogle Scholar
Littlejohn GR, Mansfield JC, Christmas JT et al (2014) An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology. Front Plant Sci 5:140PubMedPubMedCentralGoogle Scholar