Abstract
MicroRNAs (miRNAs) are functional small noncoding RNAs that work as mediators in gene silencing and that play important roles in gene regulation. A number of miRNAs have been found and their expression profiles have been examined by means of various microarray systems and real-time polymerase chain reaction (PCR) systems. Conventional microarrays as well as real-time PCR are able to detect existing miRNAs, in which inactive miRNAs that hardly contribute to gene silencing may be also contained. Here, we describe a comprehensive miRNA bioassay system with reporter genes for the detection of active miRNAs that are present in the RNA-induced silencing complexes, and actually working as mediators in gene silencing.
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Fukuoka, M., Hohjoh, H. (2018). Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells. In: Ying, SY. (eds) MicroRNA Protocols . Methods in Molecular Biology, vol 1733. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7601-0_15
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DOI: https://doi.org/10.1007/978-1-4939-7601-0_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7601-0
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