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Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime

  • Nuno Bastos
  • Sónia A. Melo
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1733)

Abstract

Exosomes are a cell-to-cell communication system that transports information to neighbor and/or distant cells. Their content is composed of lipids, proteins, RNAs, and DNA. The role of exosomes released by cancer cells in disease progression has been widely studied. Here, we report a detailed protocol to quantify precursor microRNAs and mature microRNAs overtime in exosomes of cancer cells. Those precursor microRNAs that are packaged together with RISC proteins will get further processed in the exosomes overtime and an accumulation of the mature form of the microRNAs will occur.

Key words

Exosomes Exosomes isolation Pre-microRNAs MicroRNAs qPCR 

Notes

Acknowledgments

Our laboratory is primarily supported by the project NORTE-01-0145-FEDER-000029, supported by Norte Portugal Regional Programme (NORTE 2020) under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and FEDER funds through the COMPETE Program (POCI-01/0145-FEDER-016618), as well as national funds through Foundation for Science and Technology (FCT) PTDC/BIM-ONC/2754/2014. SAM is supported by FCT IF/00543/2013.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Instituto de Investigação e Inovação em SaúdeUniversidade do PortoPortoPortugal
  2. 2.Institute of Molecular Pathology and ImmunologyUniversity of Porto (IPATIMUP)PortoPortugal
  3. 3.Medical FacultyUniversity of PortoPortoPortugal

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