Abstract
Distinct lymphocyte subpopulations display discrete metabolic profiles and are differently affected by metabolic resource variations, making the analysis of lymphocyte survival in a complex tissue in response to metabolic stress highly challenging. Here we describe a flow cytometry-based method allowing simultaneous cell identification and viable cell counting in mixed lymphocyte populations without extensive cell subset purification procedures. The example provided herein illustrates the role of AMPK in T lymphocyte survival in response to the mitochondrial poison oligomycin.
Sébastien Denanglaire and Tiphène Pirnay are Co-first authors
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Acknowledgments
The laboratory is supported by the European Regional Development Fund (ERDF), the Walloon Region (Wallonia-Biomed portfolio, 411132-957270), a Research Concerted Action of the Communauté Française de Belgique, a grant from the Fonds Jean Brachet, and a research credit from the National Fund for Scientific Research, FNRS, Belgium, and by the Belgian Program in Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister’s office, Science Policy Programming. F.A. is a Research Associate at the FNRS. T.P. was supported by a FNRS/Télévie fellowship.
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Denanglaire, S., Pirnay, T., Leo, O., Andris, F. (2018). A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress. In: Neumann, D., Viollet, B. (eds) AMPK. Methods in Molecular Biology, vol 1732. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7598-3_29
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DOI: https://doi.org/10.1007/978-1-4939-7598-3_29
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7598-3
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