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AMPK pp 229–237Cite as

Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High-Performance Liquid Chromatography

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1732))

Abstract

The serine/threonine AMP-activated protein kinase (AMPK) is a central player in the regulation of energy homeostasis, and its activity is tightly controlled, among other mechanisms, by subtle changes in cellular adenine nucleotide levels. In this chapter, we describe a step-by-step protocol for rapid, highly sensitive, reproducible, and simultaneous determination of ATP, ADP, and AMP concentrations in cell or tissue samples by reversed-phase high-performance liquid chromatography (HPLC).

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References

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Acknowledgments

Guigas’ group is supported by funding from the European Federation for the Study of Diabetes (EFSD/Lilly Research Grant Fellowship), the Société Francophone du Diabète (SFD), and the Dutch Organization for Scientific Research (ZonMW TOP Grant 91214131).

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Authors and Affiliations

  1. Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

    Noemí García-Tardón & Bruno Guigas

  2. Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

    Bruno Guigas

Authors
  1. Noemí García-Tardón
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  2. Bruno Guigas
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Corresponding author

Correspondence to Bruno Guigas .

Editor information

Editors and Affiliations

  1. Department of Pathology CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands

    Dietbert Neumann

  2. Department EMD, Inserm, U1016, Institut Cochin, CNRS, UMR8104, Université Paris Descartes, Paris, France

    Benoit Viollet

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García-Tardón, N., Guigas, B. (2018). Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High-Performance Liquid Chromatography. In: Neumann, D., Viollet, B. (eds) AMPK. Methods in Molecular Biology, vol 1732. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7598-3_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7598-3_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7597-6

  • Online ISBN: 978-1-4939-7598-3

  • eBook Packages: Springer Protocols

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