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Proteases and Cancer pp 65–72Cite as

Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1731))

Abstract

Due to their central roles in tumor growth and invasion, milligram-level amounts of active MMPs are frequently required for cancer research and development of chemical or biological MMP inhibitors. Here we describe methods for functional production of catalytic domains of MMPs (cdMMPs) in E. coli periplasm without refolding or activation process. We demonstrate applications of this straightforward approach for cdMMP-9, cdMMP-14, and cdMMP-14 mutants.

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Acknowledgments

This work was supported by National Science Foundation the Faculty Early Career Development (CAREER) Program 1453645 and National Institutes of Health Grant R01 GM115672 to X.G.

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Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA

    Dong Hyun Nam, Ki Baek Lee & Xin Ge

Authors
  1. Dong Hyun Nam
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  2. Ki Baek Lee
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  3. Xin Ge
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Corresponding author

Correspondence to Xin Ge .

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Editors and Affiliations

  1. Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Asturias, Spain

    Santiago Cal

  2. Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Asturias, Spain

    Alvaro J. Obaya

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Nam, D.H., Lee, K.B., Ge, X. (2018). Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm. In: Cal, S., Obaya, A. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 1731. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7595-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7595-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7594-5

  • Online ISBN: 978-1-4939-7595-2

  • eBook Packages: Springer Protocols

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