Abstract
Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.
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Acknowledgment
This work was supported by Genome Canada, the Natural Sciences and Engineering Research Council of Canada (NSERC), and Canada Research Chairs (CRC) programs.
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Han, W., Li, L. (2018). Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_14
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DOI: https://doi.org/10.1007/978-1-4939-7592-1_14
Publisher Name: Humana Press, New York, NY
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