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Shotgun Lipidomics Approach for Clinical Samples

  • Protocol
Clinical Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1730))

Abstract

Shotgun lipidomics offers fast and reproducible identification and quantification of lipids in clinical samples. Lipid extraction procedures based on the methyl tert-butyl protocol are well established for performing shotgun lipidomics in biomedical research. Here, we describe a shotgun lipidomics workflow that is well suited for the analysis of clinical samples such as tissue samples, blood plasma, and peripheral blood mononuclear cells.

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Abbreviations

CE:

Cholesteryl ester

Cer:

Ceramide

DAG:

Diacylglycerol

FT-ICR:

Fourier transform ion cyclotron resonance

HexCer:

Hexosyl ceramide

LPC:

Lysophosphatidylcholine

MAG:

Monoacylglycerol

MFQL:

Molecular fragmentation query language

MS:

Mass spectrometry

MS2:

Tandem mass spectrometry

MTBE:

Methyl tert-butyl ether (UPAC, tert-butyl methyl ether)

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PI:

Phosphatidylinositol

PS:

Phosphatidylserine

TAG:

Triacylglycerol

TOF:

Time of flight

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Acknowledgments

The research was supported by funds of the EXC 306 Inflammation at Interfaces, German Centre for Infection Research (DZIF TTU-TB), German Centre for Lung Research, Airway Research Center North (ARCN), and Lipidomics Informatics for Life-Sciences (LIFS) of the German Network for Bioinformatics Infrastructure (de.NBI).

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Authors and Affiliations

  1. Division of Bioanalytical Chemistry, Research Center Borstel, Borstel, 23845, Germany

    Lars F. Eggers & Dominik Schwudke

Authors
  1. Lars F. Eggers
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  2. Dominik Schwudke
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Corresponding author

Correspondence to Dominik Schwudke .

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Editors and Affiliations

  1. Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands

    Martin Giera

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Eggers, L.F., Schwudke, D. (2018). Shotgun Lipidomics Approach for Clinical Samples. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7592-1_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7591-4

  • Online ISBN: 978-1-4939-7592-1

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