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Using the Whole Cell Patch Clamp Technique to Study the Effect of Nanoparticles in Hippocampal Neurons

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Use of Nanoparticles in Neuroscience

Part of the book series: Neuromethods ((NM,volume 135))

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Abstract

Several types of nanoparticles are considered toxic to the central nervous system. Patch-clamp is one of the most indispensable techniques in the study of neuroscience, especially in the field of neurophysiology. Here, we describe the experimental details using the whole-cell patch clamp mode in the study of nanoparticles in hippocampal slices of the rat, including the generation of giga-seals and cell clamped, recording of neuronal spontaneous discharge and neuronal evoked action potentials, recording of sodium current and potassium current, and recording of glutamatergic synaptic transmission as an example. Our goal is to provide readers with guidelines on how to take the advantage of patch-clamp in the study of nanoparticles in neuroscience.

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Author information

Authors and Affiliations

  1. College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, China

    Xiaochen Zhang & Zhuo Yang

Authors
  1. Xiaochen Zhang
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  2. Zhuo Yang
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Corresponding author

Correspondence to Zhuo Yang .

Editor information

Editors and Affiliations

  1. Department of Biology, The University of Texas at San Antonio, San Antonio, Texas, USA

    Fidel Santamaria

  2. Air Force Research Laboratory, 711 HPW/RHDR, Fort Sam Houston, Texas, USA

    Xomalin G. Peralta

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Zhang, X., Yang, Z. (2018). Using the Whole Cell Patch Clamp Technique to Study the Effect of Nanoparticles in Hippocampal Neurons. In: Santamaria, F., Peralta, X. (eds) Use of Nanoparticles in Neuroscience. Neuromethods, vol 135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7584-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7584-6_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7582-2

  • Online ISBN: 978-1-4939-7584-6

  • eBook Packages: Springer Protocols

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