A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients

  • Nitesh Sule
  • Daniel Penarete-Acosta
  • Derek L. Englert
  • Arul Jayaraman
Part of the Methods in Molecular Biology book series (MIMB, volume 1729)


Microfluidic technology allows fast and precise measurement of chemotaxis responses to both attractant and repellent signals. One of the major drawbacks of current microfluidic chemotaxis assays is the presence of bacterial cells within the concentration gradient flow field, which has the potential for flow effects masking the chemotaxis response. This chapter describes a new microfluidic device for producing stable concentration gradients and measuring the response of cells to the gradient without exposing them to any flow. Unlike other methods described in the literature, this method is capable of producing gradients of any shape, almost instantaneously, allowing the measurement of time-dependent response of cells to a variety of signals.


Chemotaxis Behavioral assay Microfluidic device 



The authors would like to acknowledge partial support from the Ray B. Nesbitt Endowed Chair.


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Nitesh Sule
    • 1
  • Daniel Penarete-Acosta
    • 1
  • Derek L. Englert
    • 2
  • Arul Jayaraman
    • 1
  1. 1.Artie McFerrin Department of Chemical EngineeringTexas A&M UniversityCollege StationUSA
  2. 2.Department of Chemical and Materials EngineeringUniversity of KentuckyPaducahUSA

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