Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing
The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.
KeywordsFluorescence anisotropy Homo-FRET Chemoreceptors
The use of fluorescence anisotropy for detecting chemoreceptor responses was pioneered in the lab of Howard C. Berg . This work was funded by Israel Science Foundation.