Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1729)

Abstract

The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.

Keywords

Fluorescence anisotropy Homo-FRET Chemoreceptors 
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Notes

Acknowledgment

The use of fluorescence anisotropy for detecting chemoreceptor responses was pioneered in the lab of Howard C. Berg [5]. This work was funded by Israel Science Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.The Racah Institute of PhysicsThe Hebrew UniversityJerusalemIsrael

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