Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing

  • Vered Frank
  • Ady Vaknin
Part of the Methods in Molecular Biology book series (MIMB, volume 1729)


The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.


Fluorescence anisotropy Homo-FRET Chemoreceptors 



The use of fluorescence anisotropy for detecting chemoreceptor responses was pioneered in the lab of Howard C. Berg [5]. This work was funded by Israel Science Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.The Racah Institute of PhysicsThe Hebrew UniversityJerusalemIsrael

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