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Analyzing Chemoreceptor Interactions In Vivo with the Trifunctional Cross-Linker TMEA

  • Claudia A. Studdert
  • Diego A. Massazza
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1729)

Abstract

Chemoreceptors are dimeric proteins that contain a periplasmic or extracellular domain for ligand binding and an extremely well-conserved cytoplasmic domain for output response control. This latter domain consists in a long α-helical hairpin that forms a four-helix coiled-coil bundle in the dimer. Dimers associate into trimers of dimers in the crystal structure obtained for the cytoplasmic domain of the Escherichia coli serine chemoreceptor, Tsr. Further studies confirmed that this crystal structure reflects the basic unit within the in vivo organization of chemoreceptors. The trimers of dimers form large and stable chemoreceptor clusters in all the prokaryotes that have been studied. Here, we describe the use of TMEA, a trifunctional cross-linker that reacts with sulfhydryl groups, as a tool to study the geometry and dynamics of the interaction between receptors of the same or different types in living cells.

Keywords

TMEA Trifunctional cross-linker In vivo cross-linking Chemoreceptor interactions Trimer of dimers Cysteine reporter 

Notes

Acknowledgment

We thank Andrea Pedetta (University of Mar del Plata) for comments on the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Instituto de Agrobiotecnología del Litoral (IAL), Conicet-Universidad Nacional del Litoral, CCTSanta FeArgentina
  2. 2.Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA)Conicet-Universidad Nacional de Mar del PlataMar del PlataArgentina

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