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Bacterial Chemosensing pp 147–158Cite as

Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interface

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1729))

Abstract

Aromatic tuning facilitates stimulus-independent modulation of receptor output. The methodology is based upon the affinity of amphipathic aromatic residues, namely Trp and Tyr, for the polar–hydrophobic interfaces found within biological membranes. Here, we describe the application of aromatic tuning within the aspartate chemoreceptor of Escherichia coli (Tar). We have also employed the method within other related proteins, such as sensor histidine kinases (SHKs), and therefore hope that other research groups find it useful to modulate signal output from their receptor of interest.

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Acknowledgment

R.Y. was generously supported by the Indonesia Endowment Fund for Education, Ministry of Finance (S-4833/LPDP.3/2015). R.J.L. and L.V.E. received support from the University of Portsmouth. R.R.D. was supported with start-up funding from the Faculty of Science and from the Institute of Biological and Biomolecular Science (IBBS) at the University of Portsmouth.

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Authors and Affiliations

  1. School of Pharmacy and Biomedical Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth, UK

    Rahmi Yusuf, Robert J. Lawrence, Lucy V. Eke & Roger R. Draheim

Authors
  1. Rahmi Yusuf
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  2. Robert J. Lawrence
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  3. Lucy V. Eke
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  4. Roger R. Draheim
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Corresponding author

Correspondence to Roger R. Draheim .

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Editors and Affiliations

  1. Department of Biology, Texas A&M University, College Station, Texas, USA

    Michael D. Manson

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Yusuf, R., Lawrence, R.J., Eke, L.V., Draheim, R.R. (2018). Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interface. In: Manson, M. (eds) Bacterial Chemosensing. Methods in Molecular Biology, vol 1729. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7577-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7577-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7576-1

  • Online ISBN: 978-1-4939-7577-8

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