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Central Nervous System Neuron-Glia co-Culture Models and Application to Neuroprotective Agents

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Neurotrophic Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1727))

Abstract

Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semipermeable membrane barrier. An example of how such systems can be used to evaluate potential neuroprotective agents will also be described.

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Authors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy

    Stephen D. Skaper & Laura Facci

Authors
  1. Stephen D. Skaper
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  2. Laura Facci
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Correspondence to Stephen D. Skaper .

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Editors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stephen D. Skaper

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Skaper, S.D., Facci, L. (2018). Central Nervous System Neuron-Glia co-Culture Models and Application to Neuroprotective Agents. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7571-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7570-9

  • Online ISBN: 978-1-4939-7571-6

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