Abstract
Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic reflex. After a few days in culture, these cells reorganize to form a small group of cells that permit the generation of pacemaker activity, spontaneous contractions, and the development of inhibitory and excitatory junction potentials in the petri dish, all elements involved in the peristaltic reflex. Therefore, these co-cultures are suitable to study the cellular and molecular aspects related to the development, maintenance, and modulation of motor intestinal functions.
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This work was supported by CONACYT, México (Project No. 81409).
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Espinosa-Luna, R., Barajas-Espinosa, A.R., Ochoa-Cortez, F., Barajas-López, C. (2018). Recreating Intestinal Peristalsis in the Petri Dish. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_34
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DOI: https://doi.org/10.1007/978-1-4939-7571-6_34
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