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Growth and Neurotrophic Factors in Embryonic Stem Cells

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Book cover Neurotrophic Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1727))

Abstract

In this chapter we illustrate protocols to investigate growth and neurotrophic factors in human and rodent (rat and mouse)-derived embryonic stem cells. The conventional two-dimensional cell monolayer system to grow embryonic stem cells is presented, focusing on the coating strategies also using extracellular matrix components. Then, different approaches for three-dimensional stem cell culture are presented, using hydrogels and scaffolds. Quantitative polymerase chain reaction, immunocytochemistry, immunoenzymatic ELISA assay, and multiparametric assays to quantify growth and neurotrophic factor production are presented.

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Acknowledgments

This work has been supported by POR-FESR 2016-2020 (LC), Regione Emilia Romagna. We also acknowledge Dr. Marco Alessandri for his contribution to the experimental procedures.

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Authors and Affiliations

  1. Health Sciences and Technologies-Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Bologna, Italy

    Micaela Pannella, Luciana Giardino & Laura Calzà

  2. Department of Veterinary Medical Science, University of Bologna, Ozzano Emilia, Bologna, Italy

    Luciana Giardino & Mercedes Fernández

  3. Department of Pharmacy and Biotechnology (FaBit), University of Bologna, Bologna, Italy

    Laura Calzà

Authors
  1. Micaela Pannella
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  2. Luciana Giardino
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  3. Laura Calzà
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  4. Mercedes Fernández
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Corresponding author

Correspondence to Mercedes Fernández .

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Editors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stephen D. Skaper

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Pannella, M., Giardino, L., Calzà, L., Fernández, M. (2018). Growth and Neurotrophic Factors in Embryonic Stem Cells. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7571-6_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7570-9

  • Online ISBN: 978-1-4939-7571-6

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