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Inflammation and Cancer pp 41–52Cite as

Modelling Intestinal Carcinogenesis Using In Vitro Organoid Cultures

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1725))

Abstract

Mouse models of intestinal carcinogenesis are very powerful for studying the impact of specific mutations on tumour initiation and progression. Mutations can be studied both singularly and in combination using conditional alleles that can be induced in a temporal manner. The steps in intestinal carcinogenesis are complex and can be challenging to image in live animals at a cellular level. The ability to culture intestinal epithelial tissue in three-dimensional organoids in vitro provides an accessible system that can be genetically manipulated and easily visualised to assess specific biological impacts in living tissue. Here, we describe methodology for conditional mutation of genes in organoids from genetically modified mice via induction of Cre recombinase induced by tamoxifen or by transient exposure to TAT-Cre protein. This methodology provides a rapid platform for assessing the cellular changes induced by specific mutations in intestinal tissue.

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Acknowledgements

This work was supported by NHMRC project grants 3156594 to H.A. and 1129600 to T.J. Authors wish to acknowledge support from the Monash Flowcore and Monash Microimaging platform facilities.

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Authors and Affiliations

  1. Cancer Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

    Thierry Jardé, Genevieve Kerr, Reyhan Akhtar & Helen E. Abud

  2. The Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia

    Thierry Jardé, Genevieve Kerr, Reyhan Akhtar & Helen E. Abud

  3. Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC, Australia

    Thierry Jardé

Authors
  1. Thierry Jardé
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  2. Genevieve Kerr
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  3. Reyhan Akhtar
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  4. Helen E. Abud
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Corresponding author

Correspondence to Helen E. Abud .

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Editors and Affiliations

  1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Australia

    Brendan J. Jenkins

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Jardé, T., Kerr, G., Akhtar, R., Abud, H.E. (2018). Modelling Intestinal Carcinogenesis Using In Vitro Organoid Cultures. In: Jenkins, B. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 1725. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7568-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7568-6_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7567-9

  • Online ISBN: 978-1-4939-7568-6

  • eBook Packages: Springer Protocols

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