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Isolation and Characterization of Mouse Intrahepatic Lymphocytes by Flow Cytometry

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Inflammation and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1725))

Abstract

In the field of cellular immunology multicolor flow cytometry is a frequently applied method that allows for the simultaneously detection of multiple parameters on an individual cell basis. Flow cytometry can be used to characterize a wide range of immune cell subsets using fluorophore-conjugated antibodies to a wide range of cellular antigens. The isolation of immune cells from nonlymphoid tissue and their preparation for flow cytometry can be a challenging process with respect to immune cell yields and viability. Here we describe a method for the efficient isolation of viable mouse intrahepatic lymphocytes (IHL) from normal liver tissue and liver cancer and their subsequent characterization by multicolor flow cytometry.

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Authors and Affiliations

  1. Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC, Australia

    Florian Wiede & Tony Tiganis

  2. Peter MacCallum Cancer Centre, Melbourne, VIC, Australia

    Florian Wiede & Tony Tiganis

Authors
  1. Florian Wiede
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  2. Tony Tiganis
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Corresponding author

Correspondence to Florian Wiede .

Editor information

Editors and Affiliations

  1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Australia

    Brendan J. Jenkins

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Wiede, F., Tiganis, T. (2018). Isolation and Characterization of Mouse Intrahepatic Lymphocytes by Flow Cytometry. In: Jenkins, B. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 1725. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7568-6_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7568-6_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7567-9

  • Online ISBN: 978-1-4939-7568-6

  • eBook Packages: Springer Protocols

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