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Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease

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Book cover Inflammation and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1725))

Abstract

The gastrointestinal epithelium provides the first line of defense against invading pathogens, among which Helicobacter pylori is linked to numerous gastric pathologies, including chronic gastritis and cancer. Primary gastric epithelial cells represent a useful model for the investigation of the underlying molecular and cellular mechanisms involved in these H. pylori associated diseases. In this chapter, we describe a method for the isolation of primary gastric epithelial cells from mice and detection of epithelial cell adhesion molecule (EpCAM) expression in the isolated cells.

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Acknowledgments

This work was supported by funding from the National Health and Medical Research Council (NHMRC) to RLF (project APP1079930). Research at the Hudson Institute of Medical Research is supported by the Victorian Government’s Operational Infrastructure Support Program. RLF holds an NHMRC Senior Research Fellowship (APP1079904).

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Authors and Affiliations

  1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Melbourne, VIC, Australia

    Le Son Tran & Richard L. Ferrero

  2. Biomedicine Discovery Institute, Department of Microbiology, Monash University, Clayton, VIC, Australia

    Le Son Tran & Richard L. Ferrero

Authors
  1. Le Son Tran
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  2. Richard L. Ferrero
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Corresponding author

Correspondence to Richard L. Ferrero .

Editor information

Editors and Affiliations

  1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Australia

    Brendan J. Jenkins

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Tran, L.S., Ferrero, R.L. (2018). Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease. In: Jenkins, B. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 1725. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7568-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7568-6_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7567-9

  • Online ISBN: 978-1-4939-7568-6

  • eBook Packages: Springer Protocols

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