Abstract
In chronic lymphatic leukemia (CLL), detection and characterization of prognostic relevant chromosomal alterations is optimally done by interphase-fluorescence in situ hybridization (iFISH). Interphase nuclei derived from blood smears, bone marrow smears or from cultivated and conventionally prepared blood or bone marrow cells can be used. In CLL heterozygous or even homozygous deletion of RB1 can be found. Interestingly an iFISH diagnostic result with RB1 deletion as sole aberration is indicative for a favorable course of the disease. Here we describe the best way how to detect RB1 deletion in CLL.
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References
Jan M, Ebert BL, Jaiswal S (2017) Clonal hematopoiesis. Semin Hematol 54:43–50
Shanafelt TD (2009) Predicting clinical outcome in CLL: how and why. Hematology Am Soc Hematol Educ Program 2009:421–429
Bazargan A, Tam CS, Keating MJ (2012) Predicting survival in chronic lymphocytic leukemia. Expert Rev Anticancer Ther 12:393–403
Alhourani E, Rincic M, Othman MA, Pohle B, Schlie C, Glaser A et al (2014) Comprehensive chronic lymphocytic leukemia diagnostics by combined multiplex ligation dependent probe amplification (MLPA) and interphase fluorescence in situ hybridization (iFISH). Mol Cytogenet 7:79
Liehr T, Othman MA, Rittscher K, Alhourani E (2015) The current state of molecular cytogenetics in cancer diagnosis. Expert Rev Mol Diagn 15:517–526
Alhourani E, Aroutiounian R, Harutyunyan T, Glaser A, Schlie C, Pohle B et al (2016) Interphase molecular cytogenetic detection rates of chronic lymphocytic leukemia-specific aberrations are higher in cultivated cells than in blood or bone marrow smears. J Histochem Cytochem 64:495–501
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Liehr, T. (2018). Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia. In: Santiago-Cardona, P. (eds) The Retinoblastoma Protein. Methods in Molecular Biology, vol 1726. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7565-5_1
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DOI: https://doi.org/10.1007/978-1-4939-7565-5_1
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7565-5
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