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A Highly Efficient Strategy for Overexpressing circRNAs

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Circular RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1724))

Abstract

Circular RNAs (circRNAs) constitute an emerging class of widespread, abundant, and evolutionarily conserved noncoding RNA. They play important and diverse roles in cell development, growth, and tumorigenesis, but functions of the majority of circRNAs remain enigmatic. In order to investigate circRNA function it is necessary to manipulate its expression. While various standard approaches exist for circRNA knockdown, here we present cloning vectors for simplifying the laborious process of cloning circRNAs to achieve high-efficiency overexpression in mammalian cell lines.

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Acknowledgments

Research reported in this publication was supported by the National Health and Medical Research Council (NHMRC) project grant funding to S.J.C. (GNT1089167) and G.J.G. (GNT1089167, GNT1068773, GNT1126711). Fellowship support was provided by the Australian Research Council Future Fellowship to S.J.C. (FT160100318) and NHMRC Research Fellowship to G.J.G. (GNT1118170).

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Authors and Affiliations

  1. Centre for Cancer Biology, An Alliance Between University of South Australia and SA Health, Adelaide, SA, Australia

    Dawei Liu, Vanessa Conn, Gregory J. Goodall & Simon J. Conn

Authors
  1. Dawei Liu
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  2. Vanessa Conn
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  3. Gregory J. Goodall
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  4. Simon J. Conn
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Corresponding author

Correspondence to Simon J. Conn .

Editor information

Editors and Affiliations

  1. Department of Internal Medicine III Klaus Tschira Institute for Integrative Computational Cardiology, University Hospital Heidelberg, Heidelberg, Germany

    Christoph Dieterich

  2. Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

    Argyris Papantonis

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Liu, D., Conn, V., Goodall, G.J., Conn, S.J. (2018). A Highly Efficient Strategy for Overexpressing circRNAs. In: Dieterich, C., Papantonis, A. (eds) Circular RNAs. Methods in Molecular Biology, vol 1724. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7562-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7562-4_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7561-7

  • Online ISBN: 978-1-4939-7562-4

  • eBook Packages: Springer Protocols

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