Abstract
This chapter describes a simple and straightforward way to obtain single-stranded circular RNA sequences in vitro. Linear RNA that is phosphorylated at the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.
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Acknowledgments
This work was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).
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Abe, N., Kodama, A., Abe, H. (2018). Preparation of Circular RNA In Vitro. In: Dieterich, C., Papantonis, A. (eds) Circular RNAs. Methods in Molecular Biology, vol 1724. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7562-4_15
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DOI: https://doi.org/10.1007/978-1-4939-7562-4_15
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