Abstract
Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA.
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Acknowledgments
This work was supported by the National Basic Research Program of China (2015CB943000), National Natural Science Foundation of China (31471225), and the Fundamental Research Funds for the Central Universities (WK2070000034).
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Wang, X., Shan, G. (2018). Nonradioactive Northern Blot of circRNAs. In: Dieterich, C., Papantonis, A. (eds) Circular RNAs. Methods in Molecular Biology, vol 1724. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7562-4_11
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DOI: https://doi.org/10.1007/978-1-4939-7562-4_11
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7561-7
Online ISBN: 978-1-4939-7562-4
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