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Laser Capture Microdissection pp 371–383Cite as

A Laser Microdissection–Liquid Chromatography–Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1723))

Abstract

Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

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References

  1. Murray GI (2007) An overview of laser microdissection technologies. Acta Histochem 109:171–176

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Authors and Affiliations

  1. Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, VU University, Amsterdam, The Netherlands

    David C. Hondius, Ka Wan Li & August B. Smit

  2. Department of Pathology, Amsterdam Neuroscience, VU University Medical Center, Amsterdam, The Netherlands

    David C. Hondius, Jeroen J. M. Hoozemans & Annemieke J. M. Rozemuller

Authors
  1. David C. Hondius
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  2. Jeroen J. M. Hoozemans
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  3. Annemieke J. M. Rozemuller
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  4. Ka Wan Li
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  5. August B. Smit
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Corresponding author

Correspondence to David C. Hondius .

Editor information

Editors and Affiliations

  1. School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom

    Graeme I. Murray

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Hondius, D.C., Hoozemans, J.J.M., Rozemuller, A.J.M., Li, K.W., Smit, A.B. (2018). A Laser Microdissection–Liquid Chromatography–Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 1723. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7558-7_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7558-7_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7557-0

  • Online ISBN: 978-1-4939-7558-7

  • eBook Packages: Springer Protocols

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